So let's redraw your silica line in here.

Next, what you'd do is add in the silica.

It's hydrated dough we call the soaker that helps to start enzyme activity.

We learned how to pack a column using cotton, sand, and silica gel.

On top, you have this fine layer that you're dropping in on top of silica gel.

But when they get hydrated and they bond to each other, they create a stronger molecule, a stronger protein we call gluten.

This is now where your silica is, but as you see, the original green band has separated into two distinct bands now.

So in this case, let's say that our column was pretty crooked, what you would see instead, this is the silica line.

And as this proceeds, again you'll see the silica line, but the separation between the two bands will actually become more and more distinct.

Remember that the stationary phase is the silica gel or other material inside the column, and the mobile phase is the solvent that you pour into it.

The silica will take up most of your column, and you would be using that to fill almost all of it, just pouring it all in until it reaches a line of, say, about here.